Through the microscope

Posts tagged ‘oblique lighting’

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Rotifers with oblique lighting

I took some pictures of rotifers with oblique lighting and I’m really quite pleased with them. The cilia can be seen quite clearly and I have managed to get the rotifer’s tail in a lot of the pictures too. Rotifers have a tendency to sit on their tails.

The oblique lighting works better than phase contrast in many ways, you don’t get the halo effect with oblique that you get with phase and it is easier to get clear pictures of the cilia because of this.

Pictures taken on Vickers M4000 with oblique filter in filter tray, canon EOS 1100D camera.

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Improved onion

Improved onion

A little bit of faffing around with phase telescopes and lamps and I have much improved pictures. Not a rainbow in sight!

This time I have used oblique lighting instead of phase.
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