Here’s my onion skin, as promised. I’m not completely satisfied with the pictures. I can definitely see a rainbow across the images and I think my light must be set up wrong. I was sure I had it correct but aligning the lamp is quite tricky. The instruction book suggests using a mirror to help you position the light and to focus on the filament when setting up kohler illumination..
I don’t have the mirror that was supplied with the microscope and I haven’t managed to get any other mirror to work as suggested. I have centred my light bulb by eye as best I can but there’s a definite rainbow.
My phase rings are definitely where they should be.
I suppose they’re not too bad. I have only taken photographs a few times and I can’t expect to achieve perfection quickly. People dedicate their lives to microscopy. I have dedicated about two years in total. I have only been taking photographs for a few weeks.
I should give up working. There just isn’t enough time to do everything I want to do. I have things to sew too.
I never had this trouble with electron microscopes. I had completely different problems with them, like the sample burning up in front of you. That was always fun!
I shall have another bash at it and hopefully I shall be taking rainbow free pictures soon.